Background Information:

Measles Virus is a single negative-strand RNA virus and belongs to a member of Paramyxoviridae. Measles is caused by the measles virus, which is one of the most infectious diseases in human. Clinical measles infection can cause fever, rash and cough. It mainly affects children and young people. Measles can also cause lifelong disability, including brain damage in blindness and deafness.


Rubella virus is a single positive strand RNA, which is the only member of the Rubella virus genus. Rubella is caused by the rubella virus, which is a relatively minor infectious disease than measles. Clinical manifestations of fever and rash, prodromal symptoms are usually manifested as low fever, headache, physical discomfort, loss of appetite, cervical lymph node disease.


Measles and rubella are respiratory tract respectively caused by the measles virus and rubella virus, and it is infectious diseases characterized by acute fever rash. The prevalence of measles and rubella is similar, with a global distribution and obvious seasonal distribution characteristics, which often causes outbreaks and seriously endangers the health of children and teenagers. After the widespread use of measles attenuated virus vaccines, there are not only measles with typical symptoms in the world, but also measles with atypical symptoms which is easily confused with rubella. Laboratory testing methods include virus isolation, serological testing, and nucleic acid detection.


Bioperfectus Measles and Rubella Viruses Real-Time PCR Kit is designed to detect the measles virus and rubella virus specific RNA.



1. Detection of measles virus and rubella virus specific RNA

2. Internal Control (IC): Housekeeping gene ensures the reliability of the whole assay

3. Assay Runtime: 109 minutes

4. Limit of Detection: 10 TCID50/mL (Measles), 20 TCID50/mL (Rubella)

5. Clinical Sensitivity: 100% (Measles) 100% (Rubella)

6. Clinical Specificity: 99.5% (Measles) 99.6% (Rubella)

7. Storage Temperature: -20±5℃

8. Transportation Temperature: -25℃ ~8℃


Sample Type:

Throat swab





Measles and Rubella Viruses Real-Time PCR Kit

50 Test/Kit






Name                         Vials/Kit                    Volume (50T)            

RT-PCR Buffer               1                           375μl×1 Vial      

RT-PCR Enzyme Mix     1                           250μl×1 Vial

Reaction Mix                 1                           200μl×1 Vial      

Positive Control            1                           300μl×1 Vial      

Blank Control               1                           500μl×1 Vial      


Applicable Instrument:

ABI®7500, Roche Lightcycler®480, Bio-Rad CFX 96 Touch TM, Bioperfectus STC-96A/96A PLUS


Instruction for Use:

1.Sample Collection

2.Nucleic Acid Extraction

3.Reaction Mix Preparation

4.Run the Real-Time PCR Program

5.Get the result






Measles and Rubella Viruses Real-Time PCR Kit

Created on:2021-04-06 10:10

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